Laboratory of Food Microbiology and Food Safety, School of Veterinary Medicine, Rakuno Gakuen University

酪農学園大学　獣医学群　　　　　　　食品衛生学ユニット

069-8501
北海道江別市文京台緑町582
011-388-4723
usuima@rakuno.ac.jp

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08/03/2021 13:29
研究内容がNHKニュースで放送されました。https://www3.nhk.or.jp/sapporo-news/20210307/7000031518.html

講演情報

04/03/2021 11:15
臼井が産業動物における学び直し事業(3/26@宮崎大学)において講演を行います。

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10/02/2021 10:47

研究業績 (Publication list) を更新しました。

07/02/2021 14:32

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19/01/2021 10:22

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13/01/2021 09:16

講演情報

08/01/2021 11:18
臼井が福岡県"One Health"国際フォーラム2021（1/30〜）の分科会で講演を行います。

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04/01/2021 15:06

Publication list を更新しました。

14/12/2020 14:59

講演情報

28/09/2020 07:38
10/22; 第69回日本感染症学会東日本地方会学術集会、第67回日本化学療法学会東日本支部総会＠ウェブで臼井がシンポジウム12「環境からヒトの生活に侵入する病原微生物」を企画、座長を行いま
All articles
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Research achievements

Research paper

<2024>

1. Fukuda A, Nakajima C, Suzuki Y, Usui M*. Transferable linezolid resistance genes (optrA and poxtA) in enterococci derived from livestock composts at Japanese farms. J Glob Antimicrob Resist. 36. 336-344. 2024.

2. Enami M, Fukuda A, Yamada M, Kobae Y, Nakajima C, Suzuki Y, Usui M*. Heated scallop-shell powder and lime nitrogen effectively decrease the abundance of antimicrobial-resistant bacteria in aerobic composting. Environ Technol Innov. 34.103590. 2024.

3. Fukuda A, Suzuki M, Makita K, Usui M*. Low-frequency transmission and persistence of antimicrobial-resistant bacteria and genes from livestock to agricultural soil and crops through compost application. PloS One. 19, 30301972. 2024.

4. Sabala R, Fukuda A, Nakajima C, Suzuki Y, Usui M*, Elhadidy M*.Carbapenem and colistin-resistant hypervirulent Klebsiella pneumoniae: An emerging threat transcending the Egyptian food chain. J Infect Public Health. 17, 1037-1046. 2024.

5. Takeichi K, Fukuda A, Shono C, Ota N, Nakajima C, Suzuki Y, Usui M*. Association of toxin-producing Clostridioides difficile with piglet diarrhea and potential transmission to humans. J Vet Med Sci. 86, 769-776. 2024.

6. Koide K, Kim H, Whelan M, Belotinidos L, Tanomsridachchai T, Changkwanyeun R, Usui M, Croinin T, Thapa J, Nakajima C, Suzuki Y*. WQ-3810, a fluoroquinolone with difluoropyridine derivative as the R1 group exerts high potency against quinolone resistant Campylobacter jejuni. Microbiol Spectr. 20, e0432223. 2024.

7. Azuma T*, Usui M, Hasei T, Hayashi T. On-site Inactivation for Disinfection of Antibiotic-resistant Bacteria in Hospital Effluent by UV and UV-LED. Antibiotics. Antibiotics. 13, 711. 2024.

8. Usui M*, Azuma T, Katada S, Fukuda A, Suzuki Y, Nakajima C, Tamura Y. Hyperthermophilic composting of livestock waste drastically reduces antimicrobial resistance. Waste Manag Bullet. 2, 241-248. 2024. 

9. Makita K*, Okamura A, Fukuda A, Tokunaga T, Asai T, Shimazaki Y, Usui M. Quantitative release assessment for beta-lactamase-producing Escherichia coli of dairy origin into vegetables. CABI One Health. 3, 1. 2024.

10. Azuma T*, Usui M, Hasei T, Hayashi T. Occurrence and environmental fate of anti-influenza drugs in a subcatchment of the Yodo river Basin, Japan. Sci Total Environ. 953, 176086. 2024.

11. Okamura T, Fukuda A, Usui M*. Rapid detection of causative bacteria including multiple infections of bovine respiratory disease using 16S rRNA amplicon-based nanopore sequencing. Vet Res Commun. 2024. in press.

<2023>

1. Takeuchi H, Nakajima C, Konnai S, Maekawa N, Okagawa T, Usui M, Tamura Y, Suzuki Y, Murata S, Ohashi K. Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. Plos One. 18, e0281171. 2023.

2. Usui M*, Yoshii Y, Thiriet-Rupert S, Ghigo J M, Beloin C*.Intermittent antibiotic treatment of bacterial biofilms favors the rapid evolution of resistance. Commun. Biol. 6. 275. 2023.

3. Kakita T, Shigemura H, Fukuda A, Katamune C, Nidaira M, Kudeken T, Kyan H. Antimicrobial resistance and molecular epidemiological analysis of Escherichia fergusonii harboring the mcr gene in pigs and broiler chickens in Okinawa, Japan. J Vet Med Sci.2023. 85, 149-156. 2023

4. Usui M*, Akiyoshi M, Fukuda A, Iwano H, Kato T. 16S rRNA nanopore sequencing for rapid diagnosis of causative bacteria in bovine mastitis. Res Vet Sci.  161. 45-49. 2023.

5. Fukuda A, Tsunashima R, Usui M*. Antimicrobial resistant bacteria monitoring in retail raw seafood in Japan: A pilot study focused on Vibrio and Aeromonas. Food Safety. 2023.

6. Azuma T*, Usui M, Hayashi T. Inactivation of Antibiotic-resistant Bacteria in Hospital Wastewater by Ozone-Based Advanced Water Treatment Processes. Sci Total Environ. 2023.906. 16743. 2023.


詳細を読む: https://rakuno-gakuen-shokuei.webnode.jp/%e7%a0%94%e7%a9%b6%e6%a5%ad%e7%b8%be/ 


<2022>

1. Usui M*, Tase J, Onozaki M, Suzuki Y, Tamura Y, Nakajima C. Campylobacter Express Resistance Array for detecting the presence of fluorqouinolone- and macrolide-resistant Campylobacter jejuni and Cammpylobacter coli in broiler farms. J Appl Microbiol. 132. 3249-3255. 2022.

2. Usui M*, Fukuda A, Suzuki Y, Nakajima C, Tamura Y. Broad-host-range IncW plasmid harbouring tet(X) in Escherichia coli isolated from pigs in Japan. J Glob Antimicrob Res. 22, 97-101. 2022.

3. Kimura J, Kudo H,  Fukuda A, Yamada M, Makita K, Oka K, Takahashi M, Tamura Y, Usui M*. Decreasing the Abundance of Tetracycline-Resistant Escherichia coli in Pig Feces During Nursery Using Flavophospholipol as a Pig Feed Additive. Vet Anim Sci. 15. 100236. 2022. 

4. Fukuda A*, Nakamura H, Umeda K, Yamamoto K, Hirai Y, Usui M, Ogasawara J. Infiltration of hidden antimicrobial resistance among healthy people in a Japanese community. JAC-antimicrobial resistance. 4(3), dlac031. 2022.

5. Azuma T*, Usui M, Hayashi T. Inactivation of Antibiotic-resistant Bacteria in Wastewater by Ozone. Antibiotics. 11. 210. 2022. 

6. Gondaira S, Fujiki J, Hirano Y, Murata R, Uchida I, Usui M, Iwasaki T, Okabayashi T, Iwano H, Higuchi H*. Whole-Genome Sequencing of a Pasteurella multocida Strain Pm1 Isolated from Bovine. Microbiol Resour Announc. e0004222. 2022. 

7. Azuma T, Uchiyama T, Zhang T, Usui M, Hayashi T. Distribution and Characteristics of Carbapenem-resistant and Extended-spectrum β-lactamase (ESBL) producing Escherichia coli in Hospital Effluents, Sewage Treatment Plants, and River Water in an Urban Area of Japan. Sci Total Environ. 839. 156232. 2022.

8. Sato T, Harada K, Usui M, Yokota S, Horiuchi M. Colistin susceptibility in companion animal-derived Escherichia coli, Klebsiella spp., and Enterobacter spp. in Japan: Frequent isolation of colistin-resistant Enterobacter cloacae complex. Front Cell Infect Microbiol Bacteria Host. 12. 94841. 2022.

9. Usui M, Tamura Y, Asai T.Current Status and Future Perspective of Antimicrobial-Resistant Bacteria and Resistance Genes in Animal-Breeding Environments. J Vet Med Sci. 84. 1292-1298. 2022. 

10. Miyazato Y, Iwamoto N, Usui M, Sato T, Miyoshi-Akiyama T, Nagashima M, Maezaki K, Hayakawa K, Ohmagari N. Chromosomal coharboring of blaIMP-60 and mcr-9 in Enterobacter asburiae isolated from a Japanese woman with empyema: a case report. BMC Infect Dis. 22. 762. 2022.

11. Fukuda A, Nakano H, Suzuki Y, Nakajima C, Usui M. Conjugative IncHI2/HI2A plasmids harbouring mcr-9 in colistin-susceptible Escherichia coli isolated from diseased pigs in Japan. Access Microbiol. 4. 000454. 2022.

12. Asai T, Usui M, Sugiyama M, Andoh M. A survey of antimicrobial-resistant Escherichia coli prevalence in wild mammals in Japan using antimicrobial-containing media. J Vet Med Sci. 84. 1645-1652. 2022.

13. Fujiki J, Nakamura T, Nakamura K, Nishida K, Amano Y, Watanabe Y, Gondaira S, Usui M, Shimizu M, Miyanaga K, Watanabe S, Iwasaki T, Kiga K, Hanawa T, Higuchi H, Sawa T, Tanji Y, Tamura Y, Cui L, Iwano H. Biological properties of Staphylococcus virus ΦSA012 for phage therapy. Sci Rep. 12. 21947. 2022.

14. Kakita T, Shigemura H, Fukuda A, Katamune C, Nidaira M, Kudeken T, Kyan H. Antimicrobial resistance and molecular epidemiological analysis of Escherichia fergusonii harboring the mcr gene in pigs and broiler chickens in Okinawa, Japan. J Vet Med Sci.2022. in press.

<2021>

1.  Umeda K, Nakamura H, Fukuda A, Matsumoto Y, Motooka D, Nakamura S, Yasui Y, Yoshida H, Kawahara R. Genomic characterization of clinical Enterobacter roggenkampii co-harboring blaIMP-1- and blaGES-5-encoding IncP6 and mcr-9-encoding IncHI2 plasmids isolated in Japan. Journal of Global Antimicrobial Resistance. 24. 220-227. 2021.

2.   Tsunoda R, Usui M, Tagaki C, Fukuda A, Boonla C, Anomasiri W, Sukpanyatham N, Akapelwa ML, Nakajima C, Tamura Y, Yasuhiko Suzuki. Genetic characterization of coliform bacterial isolates from environmental water in Thailand. Journal of Infection and Chemotherapy. 27. 722-727. 2021.

3.  Usui M*, Tateno S, Onozaki M, Misawa N, Suzuki Y, Tamura Y, Nakajima C*. Rapid and Simultaneous Detection of Fluoroquinolone-and Macrolide-resistant Campylobacter jejuni/coli in Retail Chicken Meat using CAMpylobacter Express Resistance Array(CAMERA). Food Control. 123. 107815. 2021.

4.  Churak A, Poolkhet C, Tamura Y, Sato T, Fukuda A, Thongratsakul A. Evaluation of nosocomial infections through contact patterns in a small animal hospital using social network analysis and genotyping techniques. Scientific Reports. 11(1).1647. 2021.

5.  Usui M*, Nozawa Y, Fukuda A, Sato T, Yamada M, Makita K, Tamura Y. Decreased colistin resistance and mcr-1 prevalence in pig-derived Escherichia coli in Japan after banning colistin as a feed additive. Journal of Global Antimicrobial Resistance. 21. 383-386. 2021.

6.  Fukuda A*, Nakamura H, Nakamura H, Yamamoto K, Hirai Y, Usui M, Ogasawara Jun. Seven-year surveillance of the prevalence of antimicrobial-resistant Escherichia coli isolates, with a focus on ST131 clones, among healthy people in Osaka, Japan. International Journal of Antimicrobial Agents. 57(3). 106298. 2021.

7. Fukuda A, Usui M, Ushiyaam K, Shrestha D, Hashimoto N, Sakata M, Minamoto T, Yoshida O, Murakami K, Tamura Y, Asai T. Prevalence of antimicrobial-resistant Escherichia coli in the migratory bird (Anser albifrons) and their habitat in Miyajimanuma, Japan. Journal of Wildlife Diseases. 57, 954-958. 2021.

8. Odoi J, Takayanagi S, Sugiyama M, Usui M, Tamura Y, Asai T. Prevalence of Colistin-Resistant Bacteria from Retail Meats in Japan. Food Safety. 9, 48-56. 2021.

9. Nakamura K, Fujiki J, Furusawa T, Nakamura T, Gondaira S, Sasaki M, Usui M, Higuchi H, Sawa H, Tamura H, Iwano H. Complete genome sequence of Pseudomonas aeruginosa veterinary isolate, Pa12. Microbiol Resour Announc. 10, e0039821. 2021.

10. Sato T, Usui M, Harada K, Fukushima Y, Nakajima C, Szuki Y, Yokota S. Complete genome sequence of a mcr-9 possessing Enterobacter asburiae isolated from a cat in Japan. Microbiol Resour Announc. 10, e0028121. 2021.

11. Sato T, Usui M, Harada K, Fukushima Y, Nakajima C, Szuki Y, Yokota S. Complete genome sequence of a mcr-10-possessing Enterobacater roggenkampii isolated from a dog in Japan. Microbiol Resour Announc. 10, e0042621. 2021.

12. Katada S, Fukuda A, Nakajima C, Suzuki Y, Azuma T, Takei A, Takada H, Okamoto E, Kato T, Tamura Y, Usui M. Aerobic composting and anaerobic digestion decrease the copy numbers of antibiotic-resistant genes and the levels of lactose-degrading Enterobacteriaceae in dairy farms in Hokkaido, Japan. Front Microbiol.  12. 737420. 2021.

13. Nakamura K, Fujiki J, Nakamura T, Furusawa T, Gondaira S, Usui M, Higuchi H, Tamura Y, Iwano H. Bacteriophage-induced galU deficienty region is involved in trade-off effects on the phage and fluoroquinolone  sensitivity in Pseudomonas aeruginosa. Virus Res. 306:198596. 2021.

14. Kudo H, Sugiura T, Higashi S, Oka K, Takahashi T, Kamiya S, Tamura Y, Usui M. Characterization of reproductive microbiota of primiparous cows during early postpartum periods in the presence and absence of endometritis. Front Vet. 8:736996. 2021.


詳細を読む: https://rakuno-gakuen-shokuei.webnode.jp/%e7%a0%94%e7%a9%b6%e6%a5%ad%e7%b8%be/

<2020>

1.   Usui M, Maruko A, Harada M, Kawabata F, Sudo T, Noto S, Sato T, Shinagawa M, Takahashi S, Tamura Y. Prevalence and characterization of Clostridioides difficile isolates from retail food products (vegetables and meats) in Japan. Anaerobe. 61; 102131. 2020.

2.   Asai T, Usui M, Sugiyama M, Izumi K, Ikeda T, Andoh M. Antimicrobial susceptibility of Escherichia coli isolates obtained from wild animals between 2013 and 2017 in Japan. J. Vet. Med. Sci. 82,345-349.2020.

3.   Yoshizawa N, Usui M*, Fukuda A, Asai T, Higuchi H, Okamoto E, Seki K, Takada H, Tamura Y. Manure compost is a potential source of tetracycline-resistant Escherichia coli and tetracycline resistance genes in Japanese farms. antibiotics. 9(2). 2020.

4.   Thongratsaku S, Usui M, Higuchi H, Takahashi T, Sato T, Poolkhet C, Tamura Y. Prevalence and characterization of Staphylococcus aureus isolated in raw milk from cows in Hokkaido, Japan. Trop. Anim. Health Prod. 52.1631-1637.2020.

5.   Usui M. One Health approach to Clostridioides difficile in Japan. J. Infect. Chemother. 7,643-650. 2020

6.   Fukuda A, Usui M*, Tagaki C, Sukpanyatham N, Tamura Y. Escherichia coli carrying cephalosporin (bla) and colistin (mcr) resistance genes isolated from broilers and pigs in Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 51(2), 251-261. 2020.

7.   Makita K, Fujimoto Y, Miyama T, Usui M, Asai T, Ozawa M, Tamura Y. Quantinative release assessment of mcr-mediated colistin-resistant Escherichia coli from japanese pigs. Food Safety.8.13-33.2020.

8.   Sabara R, Usui M*, Tamura Y, Abd-Elghany S, Sallalm K, Elgazzar M. Prevalence of colistin-resistant Escherichia coli harbouring mcr-1 in raw beef and ready-to-eat beef products in Egypt. Food Control. 119.107436.2.020.

9.　Wakabayashi Y, Sekizuka T, Yamaguchi T, Fukuda A, Suzuki M, Kawahara R, Taguchi M, Kuroda M, Semba K, Shinomiya H, Kawatsu K. Isolation and plasmid characterisation of Salmonella enterica serovar Albany harbouring mcr-5 from retail chicken products in Japan. FEMS Microbiology Letters. 367(15), fnaa127. 2020.

10.  Fukuda A, Usui M*, Tamura Y. Roles of flies in bacterial transmission, maintenance, and contamination as vectors and reservoirs. Journal of Food: Microbiology, Safety & Hygiene. 5(1), 1-4.  2020.

11.  Yossapol M, Suzuki K, Odoi J, Sugiyama M, Usui M, Asai T. Persistence of extended-spectrum β-lactamase plasmids among Enterobacteriaceae in commercial broiler farms. Microb. Immunol.64.712-718.2020

12.  Fujiki J, Furusawa T, Munby M, Kawaguchi C, Matsuda Y, Shiokura Y, Nakamura K, Nakamura T, Sasaki M, Usui M, Iwasaki T, Gondaira S, Higuchi H, Sawa H, Tamura Y, Iwano H. Susceptibility of Pseudomonas aeruginosa Veterinary Isolates against Pbunavirus PBI-like Phages. Microb lmmunol.64. 778.-782. 2020.

13.  Munby M, Fujiki J, Aoki K, Kawaguchi C, Nakamura K, Nakamura T, Sasaki M, Sato T, Usui M, Sawa H, Yokota S, Tamura Y, Iwano H. Whole-Genome Sequence of Fluoroquinolone-Resistant Escherichia coli HUEI, Isolated in Hokkaido, Japan. Microbiol Resour Announc. 9. 46. 2020.

14.  Usui M*, Tateno S, Onozaki M, Misawa N, Suzuki Y, Tamura Y, Nakajima C*. Rapid and Simultaneous Detection of Fluoroquinolone-and Macrolide-resistant Campylobacter jejuni/coli in Retail Chicken Meat using CAMpylobacter Express Resistance Array(CAMERA). Food Control. 2021.


<2019>

1,        Okubo T, Ae R, Noda J, Iizuka Y, Usui M, Tamura Y. Detection of the sul2-strA-strB gene cluster in an ice core from Dome Fuji Station, East Antarctica. Journal of global antimicrobial resistance. 2019; 17:72-8.

2,        Kudo H, Usui M, Nagafuji W, Oka K, Takahashi M, Yamaguchi H, et al. Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum beta-lactamase and vanA genes. The Journal of antibiotics. 2019; 72:79-85.

3,        Fukuda A, Usui M, Okamura M, Dong-Liang H, Tamura Y. Role of Flies in the Maintenance of Antimicrobial Resistance in Farm Environments. Microbial drug resistance. 2019; 25:127-32.

4,        Usui M, Yokoo H, Tamura Y, Nakajima C, Suzuki Y, Jean-Marc G, Christophe B. Zinc acetate potentiate the action of tosufloxacin on Escherichia coli biofilm persisters.  Antimicrobial Agents and Chemotherapy.  24, e00069-19. 2019

5,       Usui M, Kajino A, Kon M, Fukuda A, Sato T, Shirakawa T, Kawanishi M, Harada K, Nakajima C, Suzuki Y, Tamura Y .Prevalence of 16S rRNA methylases in Gram-negative bacteria derived from companion animals and livestock in Japan. J. Vet. Med. Sci. 81, 874-878. 2019

6,       Azam A, Kadoi K, Miyanaga K, Usui M, Tamura Y, Cui L, Tanji Y. Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages. Appl. Microbiol. Biotech. 103,6809-6823. 2019.

7,      Usui M, Ozeki K, Komatsu T, Fukuda A, Tamura Y. Prevalence of extended-spectrum beta-lactamases-producing bacteria on fresh vegetables in Japan.  J. Food. Prot. 82,1663-1666. 2019. 

8,      Fukuda A, Usui M*, Masui C, Tamura Y. Quantitative analysis of houseflies-mediated food contamination with bacteria Food Safty. 7,11-14. 2019

9,     Okubo T, Hasegawa T, Fukuda A, Thapa J, Usui M, Tamura Y, Yamaguchi H. Screening of hospital-manhole sewages using MacConkey agar with cefotaxime reveals extended-spectrum β-lactamase-producing Escherichia coli. Int. J. Antimicrob. Agents. 54,831-833. 2019.

10,     Thongratsaku S, Poolkhet C, Amavisit P, Sato T, Fukuda A, Usui M, Tamura Y. Antimicrobial resistance and STEC virulence genes of Escherichia coli isolated from non-diarrheic and diaarheic dogs at a veterinary teacihng hospital in Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 50, 708-714. 2019.


<2018>

1,           Sato T, Usui M, Maetani S, Tamura Y. Prevalence of methicillin-resistant Staphylococcus aureus among veterinary staff in small animal hospitals in Sapporo, Japan, between 2008 and 2016: A follow up study. Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 2018; 24:588-91.

2,           Sato T, Shiraishi T, Hiyama Y, Honda H, Shinagawa M, Usui M, et al. Contribution of Novel Amino Acid Alterations in PmrA or PmrB to Colistin Resistance in mcr-Negative Escherichia coli Clinical Isolates, Including Major Multidrug-Resistant Lineages O25b:H4-ST131-H30Rx and Non-x. Antimicrobial agents and chemotherapy. 2018; 62.

3,           Sato T, Harada K, Usui M, Tsuyuki Y, Shiraishi T, Tamura Y, et al. Tigecycline Susceptibility of Klebsiella pneumoniae Complex and Escherichia coli Isolates from Companion Animals: The Prevalence of Tigecycline-Nonsusceptible K. pneumoniae Complex, Including Internationally Expanding Human Pathogenic Lineages. Microbial drug resistance. 2018; 24:860-7.

4,           Sato T, Fukuda A, Usui M, Shinagawa M, Shiraishi T, Tamura Y, et al. Isolation of a mcr-1-harbouring Escherichia coli isolate from a human clinical setting in Sapporo, Japan. Journal of global antimicrobial resistance. 2018; 13:20-1.

5,           Nakatsuchi A, Inagaki M, Sugiyama M, Usui M, Asai T. Association of Salmonella Serotypes with Quinolone Resistance in Broilers. Food Safety. 2018; 6:156-9.

6,        Kimura Y, Harada K, Shimizu T, Sato T, Kajino A, Usui M, et al. Species distribution, virulence factors and antimicrobial resistance of Acinetobacter spp. isolates from dogs and cats: a preliminary study. 2018; 62:462-6.

7,        Iwano H, Inoue Y, Takasago T, Kobayashi H, Furusawa T, Taniguchi K, et al. Bacteriophage PhiSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model. Biology. 2018; 7.

8,        Fukuda A, Usui M, Okubo T, Tagaki C, Sukpanyatham N, Tamura Y. Co-harboring of cephalosporin (bla)/colistin (mcr) resistance genes among Enterobacteriaceae from flies in Thailand. FEMS microbiology letters. 2018; 365.

9,        Fukuda A, Sato T, Shinagawa M, Takahashi S, Asai T, Yokota SI, et al. High prevalence of mcr-1, mcr-3 and mcr-5 in Escherichia coli derived from diseased pigs in Japan. International journal of antimicrobial agents. 2018; 51:163-4.

10,        Fujiki J, Nakamura T, Furusawa T, Ohno H, Takahashi H, Kitana J, et al. Characterization of the Lytic Capability of a LysK-Like Endolysin, Lys-phiSA012, Derived from a Polyvalent Staphylococcus aureus Bacteriophage. Pharmaceuticals (Basel, Switzerland). 2018; 11.

 

<2017>

1,        Yukawa S, Tsuyuki Y, Sato T, Fukuda A, Usui M, Tamura Y. Antimicrobial Resistance of Pseudomonas aeruginosa Isolated from Dogs and Cats in Primary Veterinary Hospitals in Japan. Jpn J Infect Dis. 2017; 70:461-3.

2,        Usui M, Kawakura M, Yoshizawa N, San LL, Nakajima C, Suzuki Y, et al. Survival and prevalence of Clostridium difficile in manure compost derived from pigs. Anaerobe. 2017; 43:15-20.

3,        Sato T, Usui M, Konishi N, Kai A, Matsui H, Hanaki H, et al. Closely related methicillin-resistant Staphylococcus aureus isolates from retail meat, cows with mastitis, and humans in Japan. PloS one. 2017; 12:e0187319.

4,        Ohishi T, Aoki K, Ishii Y, Usui M, Tamura Y, Kawanishi M, et al. Molecular epidemiological analysis of human- and chicken-derived isolates of Campylobacter jejuni in Japan using next-generation sequencing. Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 2017; 23:165-72.

5,        Harada K, Shimizu T, Mukai Y, Kuwajima K, Sato T, Kajino A, et al. Phenotypic and molecular characterization of antimicrobial resistance in Enterobacter spp. isolates from companion animals in Japan. PloS one. 2017; 12:e0174178.

6,        Fukuda A, Usui M, Wakao H, Boonla C, Tamura Y. Stenotrophomonas maltophilia is highly prevalent among houseflies (Musca domestica). Journal of medical microbiology. 2017:1202-6.

 

<2016>

1,        Usui M, Tagaki C, Fukuda A, Okubo T, Boonla C, Suzuki S, et al. Use of Aeromonas spp. as General Indicators of Antimicrobial Susceptibility among Bacteria in Aquatic Environments in Thailand. Frontiers in microbiology. 2016; 7:710.

2,        Usui M, Suzuki K, Oka K, Miyamoto K, Takahashi M, Inamatsu T, et al. Distribution and characterization of Clostridium difficile isolated from dogs in Japan. Anaerobe. 2016; 37:58-61.

3,        Sato T, Fukuda A, Suzuki Y, Shiraishi T, Honda H, Shinagawa M, et al. Pathogenic Lineage of mcr-Negative Colistin-Resistant Escherichia coli, Japan, 2008-2015. Emerg Infect Dis. 2016; 22:2223-5.

4,        Harada K, Shimizu T, Mukai Y, Kuwajima K, Sato T, Usui M, et al. Phenotypic and Molecular Characterization of Antimicrobial Resistance in Klebsiella spp. Isolates from Companion Animals in Japan: Clonal Dissemination of Multidrug-Resistant Extended-Spectrum beta-Lactamase-Producing Klebsiella pneumoniae. Frontiers in microbiology. 2016; 7:1021.

5,        Furusawa T, Iwano H, Higuchi H, Yokota H, Usui M, Iwasaki T, et al. Bacteriophage can lyse antibiotic-resistant Pseudomonas aeruginosa isolated from canine diseases. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2016; 78:1035-8.

6,        Furusawa T, Iwano H, Higuchi H, Usui M, Maruyama F, Nakagawa I, et al. Complete Genome Sequences of Broad-Host-Range Pseudomonas aeruginosa Bacteriophages PhiR18 and PhiS12-1. Genome announcements. 2016; 4.

7,        Fukuda A, Usui M, Okubo T, Tamura Y. Horizontal Transfer of Plasmid-Mediated Cephalosporin Resistance Genes in the Intestine of Houseflies (Musca domestica). Microbial drug resistance. 2016; 22:336-41.

8,        Changkwanyeun R, Yamaguchi T, Kongsoi S, Changkaew K, Yokoyama K, Kim H, et al. Impact of mutations in DNA gyrase genes on quinolone resistance in Campylobacter jejuni. Drug testing and analysis. 2016; 8:1071-6.

9,        Usui M, Shirakawa T, Fukuda A, Tamura Y. The Role of Flies in Disseminating Plasmids with Antimicrobial-Resistance Genes Between Farms. Microbial drug resistance. 2015; 21:562-9.

 

<2015>

1,        Sato T, Usui M, Motoya T, Sugiyama T, Tamura Y. Characterisation of meticillin-resistant Staphylococcus aureus ST97 and ST5 isolated from pigs in Japan. Journal of global antimicrobial resistance. 2015; 3:283-5.

2,        Changkwanyeun R, Usui M, Kongsoi S, Yokoyama K, Kim H, Suthienkul O, et al. Characterization of Campylobacter jejuni DNA gyrase as the target of quinolones. Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 2015; 21:604-9.

3,        Bien TL, Sato-Takabe Y, Ogo M, Usui M, Suzuki S. Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline. Microbes and environments / JSME. 2015; 30:339-43.

 

<2014>

1,        Usui M, Uchida I, Tamura Y. Selection of macrolide-resistant Campylobacter in pigs treated with macrolides. The Veterinary record. 2014; 175:430.

2,        Usui M, Sakemi Y, Uchida I, Tamura Y. Effects of fluoroquinolone treatment and group housing of pigs on the selection and spread of fluoroquinolone-resistant Campylobacter. Veterinary microbiology. 2014; 170:438-41.

3,        Usui M, Ozawa S, Onozato H, Kuge R, Obata Y, Uemae T, et al. Antimicrobial susceptibility of indicator bacteria isolated from chickens in southeast asian countries (Vietnam, indonesia and Thailand). The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2014; 76:685-92.

4,        Usui M, Nanbu Y, Oka K, Takahashi M, Inamatsu T, Asai T, et al. Genetic relatedness between Japanese and European isolates of Clostridium difficile originating from piglets and their risk associated with human health. Frontiers in microbiology. 2014; 5:513.

5,        Sato T, Yokota S, Okubo T, Usui M, Fujii N, Tamura Y. Phylogenetic association of fluoroquinolone and cephalosporin resistance of D-O1-ST648 Escherichia coli carrying blaCMY-2 from faecal samples of dogs in Japan. Journal of medical microbiology. 2014; 63:263-70.

6,        Sato T, Yokota S, Ichihashi R, Miyauchi T, Okubo T, Usui M, et al. Isolation of Escherichia coli strains with AcrAB-TolC efflux pump-associated intermediate interpretation or resistance to fluoroquinolone, chloramphenicol and aminopenicillin from dogs admitted to a university veterinary hospital. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2014; 76:937-45.

7,        Sato T, Okubo T, Usui M, Yokota S, Izumiyama S, Tamura Y. Association of Veterinary Third-Generation Cephalosporin Use with the Risk of Emergence of Extended-Spectrum-Cephalosporin Resistance in Escherichia coli from Dairy Cattle in Japan. PloS one. 2014; 9:e96101.

8,        Okubo T, Tosaka Y, Sato T, Usui M, Nakajima C, Suzuki Y, et al. Bacterial Diversity in Sea Ice from the Southern Ocean and the Sea of Okhotsk. Journal of Applied & Environmental Microbiology. 2014; 2:266-72.

9,        Okubo T, Sato T, Yokota S, Usui M, Tamura Y. Comparison of broad-spectrum cephalosporin-resistant Escherichia coli isolated from dogs and humans in Hokkaido, Japan. Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 2014; 20:243-9.

10,        Hiki M, Usui M, Akiyama T, Kawanishi M, Tsuyuki M, Imamura S, et al. Phylogenetic grouping, epidemiological typing, analysis of virulence genes, and antimicrobial susceptibility of Escherichia coli isolated from healthy broilers in Japan. Irish veterinary journal. 2014; 67:14.

11,        Harada K, Usui M, Asai T. Application of enrofloxacin and orbifloxacin disks approved in Japan for susceptibility testing of representative veterinary respiratory pathogens. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2014; 76:1427-30.

 

<2013>

1,        Usui M, Nagai H, Tamura Y. An in vitro method for evaluating endotoxic activity using prostaglandin E(2) induction in bovine peripheral blood. Biologicals : journal of the International Association of Biological Standardization. 2013; 41:158-61.

2,        Usui M, Nagai H, Hiki M, Tamura Y, Asai T. Effect of Antimicrobial Exposure on AcrAB Expression in Salmonella enterica Subspecies enterica Serovar Choleraesuis. Frontiers in microbiology. 2013; 4:53.

3,        Usui M, Iwasa T, Fukuda A, Sato T, Okubo T, Tamura Y. The role of flies in spreading the extended-spectrum beta-lactamase gene from cattle. Microbial drug resistance. 2013; 19:415-20.

4,        Sato T, Yokota S, Uchida I, Okubo T, Usui M, Kusumoto M, et al. Fluoroquinolone resistance mechanisms in an Escherichia coli isolate, HUE1, without quinolone resistance-determining region mutations. Frontiers in microbiology. 2013; 4:125.

5,        Sato T, Okubo T, Usui M, Higuchi H, Tamura Y. Amino acid substitutions in GyrA and ParC are associated with fluoroquinolone resistance in Mycoplasma bovis isolates from Japanese dairy calves. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2013; 75:1063-5.

6,        Hiki M, Usui M, Kojima A, Ozawa M, Ishii Y, Asai T. Diversity of plasmid replicons encoding the bla(CMY-2) gene in broad-spectrum cephalosporin-resistant Escherichia coli from livestock animals in Japan. Foodborne pathogens and disease. 2013; 10:243-9.

7,        Asai T, Usui M, Hiki M, Kawanishi M, Nagai H, Sasaki Y. Clostridium difficile isolated from the fecal contents of swine in Japan. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2013; 75:539-41.

 

<2012>

1,        Usui M, Hiki M, Murakami K, Ozawa M, Nagai H, Asai T. Evaluation of transferability of R-plasmid in bacteriocin-producing donors to bacteriocin-resistant recipients. Jpn J Infect Dis. 2012; 65:252-5.

2,        Sasaki Y, Usui M, Murakami M, Haruna M, Kojima A, Asai T, et al. Antimicrobial resistance in Shiga toxin-producing Escherichia coli O157 and O26 isolates from beef cattle. Jpn J Infect Dis. 2012; 65:117-21.

3,        Baba K, Ishihara K, Ozawa M, Usui M, Hiki M, Tamura Y, et al. Prevalence and mechanism of antimicrobial resistance in Staphylococcus aureus isolates from diseased cattle, swine and chickens in Japan. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2012; 74:561-5.

4,        Asai T, Hiki M, Baba K, Usui M, Ishihara K, Tamura Y. Presence of Staphylococcus aureus ST398 and ST9 in Swine in Japan. Jpn J Infect Dis. 2012; 65:551-2.